CONTACT INHIBITIONS OF CELL DIVISION AND CELL MOVEMENT
Top of pageAbstractInhibition of the development of dense cell cultures was studied in perfusion culture using cinemicrographic observations of rates of cell division and movement. For cell lines from normal tissues, cell division is first inhibited when a culture becomes confluent. Since depletion of the culture medium is minimized by perfusion, it is figured celltocell contact may be the primary stimulus towards the inhibition.
After a culture becomes confluent the speed of division varies either with cell motility or using the average part of the culture surface occupied with a cell, suggesting that celltocell contact affects the rate of division by restraining the area and movements from the cell surface. Thus effective inhibition of division would require adequate contact inhibition of movement (which describes the tendency of cells to avoid moving across one another) and also adequate adhesions between cell borders. It's not yet clear the way the inhibitory stimulus is transmitted to the replicative mechanism of the cell. An hypothesis in conjuction with the information is that serum macromolecules, the uptake of which is restricted to the cell surface, stimulate cell division.
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